abstract
a biosensor is an analytical device that converts a biological response into a measurable output signal. bacterial allosteric transcription factors (atfs) have been utilized as a novel class of recognition elements for in vitro biosensing, which circumvents the limitations of atf-based whole-cell biosensors (wcbs) and helps to meet the increasing requirement of small-molecule biosensors for diverse applications. in this review, we summarize the recent advances related to the configuration of atf-based biosensors in vitro . particularly, we evaluate the advantages of atfs for in vitro biosensing and highlight their great potential for the establishment of robust and easy-to-implement biosensing strategies. we argue that key technical innovations and generalizable workflows will enhance the pipeline for facile construction of diverse atf-based small-molecule biosensors.
trends in biotechnology, if="21.942
https://www.sciencedirect.com/science/article/pii/s016777992300063x
