journal of hazardous materials, 2023, https:// doi: 10.1016/j.j
abstract
mesosulfuron-methyl is a widely used herbicide in wheat fields. we previously reported that mesosulfuron-methyl alters the bacterial/fungal community structure in experimental indoor microcosms, ultimately affecting no3--n and nh4 -n contents in soil nitrogen (n) cycling. however, how mesosulfuron-methyl application alter soil n cycling by changing microbial community assembly is unknown. here, we designed an outdoor experiment comprising 2-month periods to investigate changes in soil n-cycle functional genes and structural shifts in the microbial community assembly in response to mesosulfuron-methyl applied at 11.25 and 112.5 g a.i. hm-2. results showed that high mesosulfuron-methyl input significantly decreased aoa amoa and nirk abundances within the initial 15 days, but increased aob amoa on day 60. the nifh abundance displayed a stimulation-inhibition trend. moreover, high mesosulfuron-methyl input decreased the network's complexity, and newly formed multiple network modules exhibited strong negative associations with nifh, aob amoa, nirk and nirs . further structural equation model demonstrated that mesosulfuron-methyl did reveal strong direct inhibition of nirk, and it indirectly affected nirk by changing nifh abundance and planomicrobium . thus mesosulfuron-methyl perturbs n-cycling processes by reshaping bacterial community assembly. taken together, our study provides theoretical support for determining the microbiological mechanism by which mesosulfuron-methyl affects soil n cycling.
journal of hazardous materials,if=13.6
