nature communications,
abstract
proteins containing a ubiquitin regulatory x (ubx) domain are cofactors ofcell division cycle 48 (cdc48) and function in protein quality control. how-ever, whether and how ubx-containing proteins participate in host–microbeinteractions remain unclear. here we show that monle1, an effector from thefungal pathogen magnaporthe oryzae, is a core virulence factor that sup-presses rice immunity by specifically interfering with ospux8b.2. the ubxdomain of ospux8b.2 is required for its binding to osatg8 and oscdc48-6and controls its 26 s proteasome–dependent stability. ospux8b.2 andoscdc48-6 positively regulate plant immunity against blast fungus, while thehigh-temperature tolerance heat-shock protein osbht, a putative cytoplasmicsubstrate of ospux8b.2–oscdc48-6, negatively regulates defense againstblast infection. monle1 promotes the nuclear migration and degradation ofospux8b.2 and disturbs its association with osbht. given the high con-servation of monle1 among fungal isolates, plants with broad and durableblast resistance might be generated by engineering intracellular proteinsresistant to monle1.
nature communications，if=16
