张杰,研究员,博士生导师,中国农业科学院植物保护研究所副所长。1987年、1990年在原北京农业大学植物生理生化专业分别获得理学学士、硕士学位。2000年毕业于中国农业科学院研究生院,获农学博士学位。2002-2003年美国cornell大学从事博士后研究工作。先后兼任植物保护学会生物安全专业委员会副主任,中国生化与分子生物学学会农业分会常务理事,国际无脊椎动物病理学会细菌分会理事,《中国生物防治学报》主编,微生物学报》等刊物编委。
一直从事bt菌株、基因资源挖掘与应用、蛋白杀虫机制研究,先后、863课题、转基因生物新品种培育重大专项、国家重点研发计划项目和国家自然科学基金等20余个项目;相关研究成果申请国家发明专利,已有38项获得授权,其中一项专利获第十八届中国专利优秀奖;在国内外重要期刊发表学术论文200余篇,其中sci源论文90篇。曾获农业部中华农业科技奖—优秀创新团队奖、大北农科技成果奖,获得农业部“农业科研杰出人才”称号。
针对我国重要农业害虫,基于高效的新基因克隆及鉴定平台,获得了一系列针对鳞翅目、鞘翅目和刺吸式害虫高毒力的bt新基因;构建了广杀虫谱、无芽胞、生物微胶囊等不同特性的工程菌株,获得了国际第一个鳞翅目、鞘翅目兼杀的bt工程菌g033a,也是我国首个获得农药登记证的防治鞘翅目害虫的bt菌株;先后与中科院遗传所、中国农大等7家科研单位及奥瑞金、中国种子集团、美国杜邦先锋等国内外公司合作,构建转基因抗虫作物,为我国bt生物农药的应用及转基因抗虫作物的研发做出了应有的贡献。
近五年作为通讯作者发表的代表性论文:
1、jiang jian, huang ying, shu changlong, soberón mario, bravo ale, chunqin liu, song fuping, jinsheng lai, jie zhang*. 2017. holotrichia oblita midgut proteins that bind to bacillus thuringiensis cry8-like toxin and assembly of the h. oblita midgut tissue transcriptome. applied and environmental microbiology. 31: 83(12).
2、zishan zhou, yuxiao liu, gemei liang, yongping huang, alejandra bravo, mario soberón, fuping song, xueping zhou, jie zhang*. 2017. insecticidal specificity of cry1ah to helicoverpa armigera is determined by binding apn1 through domain ii loops 2 and 3. applied and environmental microbiology. 83:e02864-16.
3、chongsi sun, lili geng, meiling wang, gaoxiang shao, yongfeng liu, changlong shu, jie zhang*. 2017. no adverse effects of transgenic maize on population dynamics of endophytic bacillus subtilis strain b916-gfp. microbiologyopen . 6:e00404.
4、yan-qiu li, changlong shu, yueming shan, lili geng, fuping song, jie zhang*. 2017. complete genome sequence of bacillus thuringiensis bt185, a potential soil insect biocontrol agent. journal of integrative agriculture . 16(3): 749–751.
5、fengjiao zhang, changlong shu, neil crickmore, yanqiu li, fuping song, chunqin liu, zhibao chen, jie zhang*. 2016, use of redundant exclusion pcr to identify a novel bacillus thuringiensis cry8 toxin gene from pooled genomic dna. applied and environmental microbiology. 82(13): 3808-15.
6、changlong shu, jianqiao zhou, neil crickmore, xianchun li, fuping song, gemei liang, kanglai he, dafang huang, jie zhang*. 2016. in vitro template-change pcr to create single crossover libraries: a case study with b. thuringiensis cry2a toxins. scientific reports . 6: 23536, doi: 10.1038.
7、zishan zhou, zeyu wang, yuxiao liu, gemei liang, changlong shu, fuping song, xueping zhou, alejandra bravo, mario soberón, jie zhang*. 2016. identification of abcc2 as a binding protein of cry1ac on brush border membrane vesicles from helicoverpa armigera by an improved pull-down assay. microbiologyopen. 5(4): 659–669.
8、longfa fang, bowang, zishan zhou, sujuan yang, changlong shu, fuping song, alejandra bravo, mario soberón, jie zhang*. 2016. oligomerization of cry9aa in solution without receptor binding, is not related with insecticidal activity. electronic journal of biotechnology . 21: 54–57.
9、yang bi, yanrui zhang, changlong shu, neil crickmore, qinglei wang, lixin du, fuping song, and jie zhang*. 2015. genomic sequencing identifies novel bacillus thuringiensis vip1/vip2 binary and cry8 toxins that have high toxicity to scarabaeoidea larvae. applied microbiology and biotechnology . 99(2): 753-760.
10、changlong shu, shuqian tan, jiao yin, mario soberón, alejandra bravo, chunqing liu, lili geng, fuping song, kebin li and jie zhang*. 2015. assembling of holotrichia parallela (dark black chafer) midgut tissue transcriptome and identification of midgut proteins that bind to cry8ea toxin from bacillus thuringiensis. applied microbiology and biotechnology . 99(17):7209-7218.
11、, , , , and *. 2015. identification of a new cry1i -type gene as a candidate for gene pyramiding in corn to control ostrinia species larvae. applied and environmental microbiology . 81(11): 3699-3705.
12、zisan zhou, sujuan yang, shu changlong shu, fuping song, xueping zhou, jie zhang*. comparison and optimization of the method for cry1ac protoxin preparation in hd73 strain. 2015. journal of integrative agriculture . 14(8): 1598–1603.
13、lili geng, xiaohong duan, chun liang, changlong shu, fuping song, jie zhang*. 2014. mining tissue-specific contigs from peanut ( arachis hypogaea l.) for promoter cloning by deep transcriptome sequencing. plant cell physiology. 55(10): 1793–1801.
14、ying li, changlong shu, xuewen zhang, neil crickmore, gemei liang, xingfu jiang, rongmei liu, fuping song, jie zhang*. 2014. mining rare and ubiquitous toxin genes from a large collection of bacillus thuringiensis strains. journal of invertebrate pathology. 122: 6–9.
15、zishan zhou, huiyan lin, ying li, changlong shu, fuping song and jie zhang*. 2014. the minimal active fragment of the cry1ai toxin is located between 36i and 605i. journal of integrative agriculture , 13(5): 1036-1042.
16、yueming shan, changlong shu, neil crickmore, chunqin liu, wensheng xiang, fuping song, jie zhang*. 2014. cultivable gut bacteria of scarabs (coleoptera: scarabaeidae) inhibit bacillus thuringiensis multiplication. environmental entomology . 43(3): 612-616.
17、yanhua jia,• can zhao, qinglei wang, changlong shu, xiaojie feng, fuping song, jie zhang*. 2014. a genetically modified broad-spectrum strain of bacillus thuringiensis toxic against holotrichia parallela , anomala corpulenta and holotrichia oblita . world journal of microbiology & biotechnology. 30(2): 595-603.
18、changlong shu, dongming liu, zishan zhou, jilin cai, qi peng, jiguo gao, fuping song and jie zhang*. 2013. an improved pcr-rflp method for the identification of cry1 -type genes. applied and environmental microbiology. 9(21): 6706-6711.
19、lili geng, jing chi, changlong shu, peter gresshoff, fuping song, dafang huang, jie zhang*. 2013. a chimeric cry8ea1 gene flanked by mars efficiently controls holotrichia parallela . plant cell reports. 32: 1211–1218.
20、weiyu jiang, lili geng, pingli dai, zhihong lang, changlong shu, yi lin, ting, zhou, fuping song and jie zhang*. 2013. the influence of bt-transgenic maize pollen on the bacterial diversity in the midgut of chinese honeybees, apis cerana cerana. journal of integrative agriculture . 12(3): 474-482.