李广悦，中国农业科学院植物保护研究所研究员, 博士生导师, 中国农业科学院“生物杀菌创制与应用”创新团队首席科学家，中国农业科学院青年英才“a”类入选者。2003-2007年就读于河北农业大学生命学院并获得学士学位，2007-2010年就读于中国农业科学院植物保护研究所并获得硕士学位，2010-2014年就读于中国科学院天津工业生物技术研究所并获得博士学位。2014-2017年在德国马普煤炭所manfred.t.reetz教授实验室从事博士后研究。2017年11月回国，入职中国农业科学院植物保护研究所。主要从事线虫共生细菌中抗菌活性物质的发掘、代谢途径优化以及代谢途径中关键酶的定向改造等研究。在jacs、acs catalysis、chemical science、chemical communications等学术期刊上发表文章20多篇。
已发表文章
1.li, g#.; qin, y#.; fontaine, n. t#.; ng fuk chong, m.; maria‐solano, m. a.; feixas, f.; cadet, x. f.; pandjaitan, r.; garcia‐borràs, m.; cadet, f., machine learning enables selection of epistatic enzyme mutants for stability against unfolding and detrimental aggregation. chembiochem . 2020, 21,1-12. (if5year ="2.5)
2. 李广悦, 杨秀芬. 嗜线虫致病杆菌抗菌代谢产物 xcn1 的研究进展. 中国生物防治学报 2020, 36 (1), 1-8.
3. 段佳琪，李琲琲，任杰，邱德文，李广悦*.一种脱氧雪腐镰刀菌烯醇制备工艺的研究.生物技术通报. 2020, 36(10):1-8.
4. dong, y.; li, x.; duan, j.; qin, y.; yang, x.; ren, j.; li, g*. improving the yield of xenocoumacin 1 enabled by in situ product removal. acs omega. 2020, 5（32）, 20391–20398. (if5year ="2.6)
5. jiaqi duan, beibei li, youcai qin, yijie dong, jie ren and guangyue li*. recent progress in directed evolution of stereoselective monoamine oxidases. bioresources and bioprocessing [j]. 2019, 6: 1-11.
6.guangyue li, yijie dong, and manfred t. reetz*. can machine learning revolutionize directed evolution of selective enzymes? adv. synth. catal [j]. 2019, 361, 2377 – 2386. (if5year ="5.029)
7.yijie dong#, peiyuan yao#, yunfeng cui, qiaqing wu, dunming zhu*, guangyue li*and manfred t. reetz*. manipulating the stereoselectivity of a thermostable alcohol dehydrogenase by directed evolution for efficient asymmetric synthesis of arylpropanols. biological chemistry [j]. 2019，400(3), 313-321. (if5year ="3.2)
8.guangyue li, marc garcia-borràs, maximilian j. l. j. fürst, adriana ilie, marco w. fraaije, k. n. houk*and manfred t. reetz*. overriding traditional electronic effects in biocatalytic baeyer-villiger reactions by directed evolution. jacs [j]. 2018, 140, 10464−10472. (if5year ="14.35)
9.peiyuan yao, peiqian cong, rui gong, jinlong li, guangyue li, jie ren, jinhui feng, jianping lin, peter c. k. lau,* qiaqing wu,* and dunming zhu*.biocatalytic route to chiral 2-substituted-1, 2, 3, 4-tetrahydroquinolines using cyclohexylamine oxidase muteins. acs catalysis[j]. 2018, 8, 1648-1652. (if5year ="10.945).
10.frédéric cadet, nicolas fontaine, guangyue li, joaquin sanchis, matthieu ng fuk chong,rudy pandjaitan, iyanar vetrivel , bernard offmann & manfred t. reetz. a machine learning approach for reliable prediction of amino acid interactions and its application in the directed evolution of enantioselective enzymes. scientific reports [j]. 2018, 8:16757. (if5year ="4.12)
11.ge qu, richard lonsdale, peiyuan yao, guangyue li, beibei liu, manfred t. reetz,* and zhoutong sun*. methodology development in directed evolution: exploring options when applying triple‐code saturation mutagenesis. chembiochem[j]. 2018, 19, 239-246. (if5year ="2.751).
12.guangyue li#, peiyuan yao#, rui gong#, jinlong li, pi liu，richard lonsdale, qiaqing wu, jianping lin*，dunming zhu* and manfred t. reetz*. simultaneous engineering of an enzyme’s entrance tunnel and active site: the case of monoamine oxidase mao-n. chemical science [j]. 2017, 8，4093-4099. (if5year="8.733)
13.guangyue li, miguel a. maria-solano, adrian romero-rivera, sílvia osuna* and manfred t. reetz*. inducing high activity of a thermophilic enzyme at ambient temperatures by directed evolution. chemical communications [j]. 2017, 53，9454-9457. (if5year ="6.238)
14.guangyue li, m.j. l. j. fürst, h. r. mansouri, a. k. ressmann, a. ilie, f. rudroff, m. d. mihovilovic*, m.w. fraaije* and m. t. reetz*. manipulating the stereoselectivity of the thermostable baeyer–villiger monooxygenase tmchmo by directed evolution. organic & biomolecular chemistry [j]. 2017，15，9824-9829. (if5year ="3.56)
15.guangyue li, jianbo wang and manfred t. reetz*. biocatalysts for the pharmaceutical industry generated by structure-guided directed evolution ofstereoselective enzymes. bioorganic & medicinal chemistry [j]. 2017, 7(26), 1241-1251 (if5year="2.88)
16.jian-bo wang, guangyue li, manfred t. reetz*. enzymatic site-selectivity enabled by structure-guided directed evolution. chemical communications [j]. 2017, 53, 3916-3928. (if5year ="6.238)
17.guangyue li#, hui zhang#, zhoutong sun, xinqi liu and manfred t. reetz*. multiparameter optimization in directed evolution: engineering thermostability, enantioselectivity, and activity of an epoxide hydrolase. acs catalysis [j]. 2016, 6(6), 3679−3687. (if5year ="10.945)
18.guangyue li and manfred t. reetz*. learning lessons from directed evolution of stereoselective enzymes. org. chem. front [j]. 2016. 3, 1350-1358 (if5year ="4.96)
19.guangyue li#, peiyuan yao#, peiqian cong, jie ren, lei wang, jinhui feng, peter c.k. lau, qiaqing wu*,and dunming zhu*. new recombinant cyclohexylamine oxidase variants for deracemization of secondary amines by orthogonally assaying designed mutants with structurally diverse substrates. scientific reports [j]. 2016，6：24973. (if5year ="4.847)
20.zhoutong sun†, guangyue li†, adriana ilie†, manfred t. reetz *. exploring the substrate scope of mutants derived from the robust alcohol dehydrogenase tbsadh. tetrahedron letters[j]. 2016. 57, 3648-3651. (if5year ="2.02).
21.zhoutong sun, richard lonsdale, adriana ilie, guangyue li, jiahai zhou, and manfred t. reetz. catalytic asymmetric reduction of difficult-to-reduce ketones:triple-code saturation mutagenesis of an alcohol dehydrogenase. acs catalysis [j]. 2016, 6(3)， 1598−1605. (if5year ="10.945)
22.zhoutong sun, richard lonsdale, lian wu, guangyue li, aitao li, jianbo wang, jiahai zhou, and manfred t. reetz. structure-guided triple-code saturation mutagenesis: efficient tuning of the stereoselectivity of an epoxide hydrolase. acs catalysis [j]. 2016, 6， 1590−1597. (if5year ="10.945)
23.zhoutong sun, richard lonsdale, guangyue li and manfred t. reetz. comparing different strategies in directed evolution of enzyme stereoselectivity: single versus double code saturation mutagenesis. chembiochem [j]. 2016, 17， 1865-1872. (if5year ="2.751)
24.guangyue li#, jie ren#, peiyuan yao,yitao duan, hailing zhang, qiaqing wu, jinhui feng, peter ck lau*, dunming zhu*. deracemization of 2-methyl-1, 2, 3, 4-tetrahydroquinoline using mutant cyclohexylamine oxidase obtained by iterative saturation mutagenesis. acs catalysis [j]. 2014, 4，903-908. (if5year ="9.174)
25.guangyue li, jie ren, hiroaki iwaki, dalong zhang, yoshie hasegawa, qiaqing wu, jinhui feng, peter c. k. lau* and dunming zhu*. substrate profiling of cyclohexylamine oxidase and its mutants reveals new biocatalytic potential in deracemization of racemic amines. applied microbiology and biotechnology [j]. 2014, 98(4)，1681-1689. (if5year ="3.848)
26.guangyue li, jie ren, qiaqing wu*, jinhui feng, dunming zhu*, yanhe ma. identification of a marine nadph-dependent aldehyde reductase for chemoselective reduction of aldehydes. journal of molecular catalysis b, enzymatic [j], 2013, 90，17-22. (if5year ="2.786)
27.li guangyue，yang xiufen，zeng hongmei，mao jianjun，liu hua，zhang yunhua，qiu, dewen* stable isotope labelled mass spectrometry for quantification of the relative abundances for expressed proteins induced by peat1. science china life sciences [j], 2010, 53(12)，1410-7. (近五年平均if=2.88)
28.梁颖博, 李泽, 邱德文, 曾洪梅, 李广悦, 杨秀芬. 本生烟响应蛋白激发子 pevd1 的差异表达基因鉴定与分析. 中国农业科学, 2019，52(21), 3794-3805.
29.刘 权, 李广悦, 曾洪梅, 杨秀芬, 邱德文. 微生物蛋白激发子peat1的获得及诱导水稻抗旱性的初步研究. 中国农业科技导报, 2009, 11( 3)， 51- 55.
30.刘权，李广悦，曾洪梅，杨秀芬，邱德文．蛋白激发子peat1的高密度发酵及生理功能检测．生物技术通报，2009，8，162-165．
